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產品資訊

高解析度數位細胞/組織電生理訊號放大器 dPatch Digital Patch Clamp Amplifier

產品型號: dPatch
廠牌名稱: Sutter Instruments
Sutter Instruments 匯集了各家傳統電生理儀器廠牌的優點,並且開發出了dPatch® 數位細胞/組織電生理訊號放大器。此款數位放大器擁有低噪音、高頻寬與精準的特性,解析度高達 22bit 並且能夠一機同時進行兩種不同的電生理實驗 (Voltage clamp 或是 Current clamp) 帶給您絕佳的電生理實驗數據及絕佳的操作體驗。

產品特點

產品特色

  • 完整的售後服務與實驗技術指導
  • 一套設備即包含所有電生理記錄所需的硬體,安裝非常簡單且已經包含了1個或2個前端訊號放大器 (Headstage)
  • 從單一細胞記錄到組織切片記錄皆可使用,無需更換設備
  • 不論何種電生理記錄,電極阻抗皆可透過電腦進行全自動調整
  • 接收頻率、波段過濾、放大倍率皆可透過電腦控制
  • 訊號補償與低雜訊的硬體設計帶給您清楚且不失真的電生理訊號
  • 包含了功能強大的SutterPatch® 記錄分析軟體,賦予您充足的數據記錄/分析彈性

AMPLIFIER SYSTEMS

Sutter Instrument is proud to announce one of the most significant additions to our product lineup in years: a full suite of electrophysiology recording hardware and software.

The dPatch® Digital Patch Amplifier System combines an unmatched sampling rate of up to 5 MHz with noise performance that supports the quietest single-channel recordings and digital architecture for the highest signal fidelity and ultra-stable compensation circuitry. The dPatch represents the most advanced amplifier for electrophysiology on the market today.

The IPA® family of Integrated Patch Amplifiers, enables efficient, low-noise whole-cell recordings. The IPA system, available with one (IPA) or two recording channels (Double IPA), combines state-of-the-art amplifier technology with fully integrated D/A and A/D conversion and a high speed USB interface. Acquisition, data management, and streamlined analysis are performed using the bundled SutterPatch® Data Acquisition and Analysis Software, built on the foundation of Igor Pro 8 (WaveMetrics, Inc.).

All Sutter Amplifier Systems were designed with the attention to detail our customers expect and appreciate. The faceplates are sculpted and feature recessed connectors. The pipette holder connects to machined aluminum threads designed for the utmost in mechanical stability and improved shielding. Available accessories include expansion panels, which present amplifier back panel connections at the front of a 19” rack; a machined brass ground point; standard pipette holders made of polycarbonate; premium pipette holders made of quartz, which minimizes thermal expansion (now standard on dPatch Systems).

dPatch® Digital Patch Clamp Amplifier

Sutter Instrument is proud to introduce the latest cutting-edge tool for electrophysiology research, the dPatch® Digital Patch Clamp Amplifier. Using high-speed digital processing, matched with high-resolution data converters and paired with SutterPatch® software’s intuitive user interface, this low-noise, high-bandwidth precision instrument provides capabilities previously unavailable to electrophysiologists.

Available in either a single- or dual-headstage configuration, the dPatch amplifier’s architecture makes swapping headstages, or adding a second one to a single-headstage version, a plug-and-play operation. The two headstages are independently configurable for either voltage clamp or true high-speed current clamp. Data is streamed from each headstage in parallel and fully synchronized.

 

5 MHz SAMPLING RATE, UP TO 22-BIT RESOLUTION

One unique feature with dPatch is the headstage data sampling system. Each headstage is continually sampled at 5 MHz. Output filtering has thirteen settings between 100 Hz and 1 MHz. A resolution of 18 bits is achieved at 1 MHz. For lower filter settings, automatic downsampling increases resolution while optimizing data rates. At a bandwidth setting of 1 kHz, the dPatch system provides a signal resolution of better than 22 bits.
 

OPTIMAL NOISE PERFORMANCE IN BOTH THE CAPACITIVE AND RESISTIVE FEEDBACK RANGES

When other products remove thermal energy from the headstage circuitry, it in turn heats up the headstage housing. That leads to thermal drift of the pipette and other, cumbersome side effects. One of the development goals of the dPatch headstage was achieving a comparable noise performance at room temperature, without the need for a cooled headstage. In the two resistive feedback modes, the dPatch amplifier is even quieter than any of the competitor systems.
 

BUILT-IN DATA ACQUISITION SYSTEM MEANS NO THIRD-PARTY DIGITIZER

Using a multiplexer-free design, the dPatch provides 8 fully differential analog input channels, 4 analog output channels, and 16 digital outputs (TTL). All I/O channels are sampled continuously (200 kHz for analog inputs, 250 kHz for analog and digital outputs) and available through the user interface.
 

SUTTERPATCH SOFTWARE

The dPatch amplifier, in combination with SutterPatch software, has been engineered to automatically capture and store all amplifier settings, stimulus information and external experiment parameters, and associate them in time with the raw data traces. This includes all amplifier and acquisition settings, as well as timing and progress of the experiment. Fully integrated computer control of the amplifier stages means that the acquisition software is aware of the internal state of the amplifier and digitizer at all times and can track any changes that may occur. This is independent of whether a change is triggered automatically or initiated by the user.
 

TRACKING OF OTHER EXTERNAL DATA

In addition to status changes in connected hardware that are automatically tracked, the researcher can manually trigger tags to document events like stimulus application using instruments not connected to the amplifier.

Information about environmental parameters and a more detailed specification of sample properties can be recorded and stored with the raw data. A total of over 600 metadata attributes are supported. Examples include: animal species, genotype, date/time when a cell sample was prepared, recording solutions, pipette resistance, hardware properties, and detailed information about stimuli applied.

 

DATA VISUALIZATION AND ANALYSIS

SutterPatch software has been designed to simplify the navigation and analysis of complex datasets. The scope window supports multiple view modes in both two-dimensional and an innovative three-dimensional display. The 3D view is particularly useful during assay development. Built on top of the latest version of the proven Igor Pro platform, SutterPatch combines native Igor Pro functionality with a wealth of features that are tailored to electrophysiology applications. Both the newcomer and the experienced user of patch clamp programs will feel comfortable using SutterPatch software.
 

Application modules provide focused functionality for particular applications.

Currently available:

  • Event Detection Module: A deconvolution algorithm that excels at detecting miniature synaptic events even on a noisy background.
  • Action Potential Analysis Module: Phase plane plot, timing and waveform statistics.
  • Camera Module: An easy way to document the identity and condition of the recorded cell.

 

A LABORATORY WORKHORSE

While the dPatch System is ready for cutting-edge research, its feature set makes it immediately valuable in any lab setting.

  • Three headstage feedback ranges for optimal whole-cell and single-channel recording
  • Automated compensation of electrode and whole-cell capacitance
  • Series resistance compensation
  • Simple cabling, quick and easy set-up
  • High dynamic range of digitizer means no need for variable gain stages
  • High speed of digitizer means no concern about sample rate


COMMON APPLICATIONS:

  • Single-channel recordings
  • Auditory research and other rapidly changing signals
  • Tissue slice recordings
  • Cultured cell experiments
  • Cell line studies from adherent or dispersed cells
  • Optogenetics

FEATURES

  • Fully integrated single- or dual-headstage patch clamp amplifier and data acquisition system ensures quick and easy setup
  • Optimized for whole-cell patch clamp recordings in tissue slices, adherent or dissociated cells
  • Full computer control provides automated compensation of electrode and whole cell capacitance
  • Voltage and true current clamp capability for complete characterization of cells' electrical activity
  • Line frequency reduction in SutterPatch
  • High bandwidth enables characterization of the fastest signals
  • Three headstage feedback ranges for single-channel and whole-cell patch clamp recordings
  • Comprehensive digital compensation circuitry provides the utmost precision and signal fidelity
  • Bundled SutterPatch® software provides versatile data management, intuitive navigation and streamlined data analysis.

 

ACCESSORIES

Ground Point
The Ground Point GP-17 provides reliable, low resistance connections for a star ground configuration, the proven method to avoid ground loops in any electrophysiology setup. Accepts 9 banana plugs + 8 bare wires up to 10 gauge or banana plugs. The baseplate mounts directly on imperial or metric air table tops with the included ¼-20 and M6 screws. Made of solid, machined brass with plated banana/clamp connectors.



dPatch Expansion Panel

產品規格

The dPatch Integrated Digital Patch Clamp Amplifier is a computer-controlled single- or dual-headstage system optimized for both single-channel and whole-cell recording applications.


back panel of dPatch. (Click for larger view)

 

Amplifier

Feedback
Element
Range
Analog
Bandwidth
Noise
10 kHz BW
Pipette
Compensation
Range
Series
Resistance
Range
Cell
Capacitance
Range
Capacitive
±20 nA
> 500 kHz
< 0.22 pArms
20 pF
N/A*
N/A*
500 MΩ
±20 nA
> 250 kHz
< 0.7 pArms
20 pF
100 MΩ
100 pF
50 MΩ
±200 nA
> 250 kHz
< 2.3 pArms
20 pF
10 MΩ
1000 pF
  • Hardware architecture enables all data conversion to be performed near the preparation, well away from known noise sources, such as power supplies and high-speed digital circuitry.
  • Voltage clamp and true current clamp modes with smart switching between modes to avoid current artifacts
  • Three choices of headstage feedback elements to optimize both single-channel and whole-cell recording
  • * Capacitive feedback range is optimized for single-channel voltage clamp recordings. Whole-cell compensation and current clamp mode are disabled with this range.

 

  • Automatic compensation routines for pipette compensation and whole-cell compensation, and series resistance compensation
  • Series resistance prediction and correction independently programmable
  • 8-pole Bessel filter 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100, 250, 500, 1000 kHz
  • Signal processing of filter output to increase resolution and reduce data file size
  • Resolution over 22 bits at 1 kHz filter setting
  • High dynamic range of analog to digital converters alleviates need for variable output gain stages
  • Holding potential ±1000 mV
  • Current clamp bridge compensation and pipette capacitance compensation
  • Slow holding potential tracking compensates for drift during current clamp recordings

 

Data Acquisition

  • Embedded data acquisition system eliminates the need for an external data acquisition board
    • 5 MHz sampling rate per headstage, 22-bit resolution
    • 8 Auxiliary analog inputs, 16-bit fully differential, ±10 V input, each continuously sampled at 200 kHz
    • 4 Analog outputs, 16-Bits, ±10 V output each continuously updated at 250 kHz
    • 16 Digital outputs (TTL) each running at 250 kHz
    • Independent Trigger IN / Trigger OUT for synchronization of external instrumentation
  • Single SuperSpeed USB 3.0 connection controls data acquisition and amplifier
  • Complex command waveforms
  • Data acquisition can be initiated by an onboard microsecond clock or external (TTL) trigger

 

SutterPatch Software

  • Built on the foundation of Igor Pro 8 (WaveMetrics, Inc.)
  • Paradigms and Routines provide complete experimental control
  • Waveform Editor for easy creation of even the most complex stimulus patterns or user-defined templates
  • Associated Metadata stores all relevant information regarding your experiment
  • Comprehensive data analysis routines and publication-quality graphics
  • Rapid-response online line-frequency reduction
  • Runs on Windows 10 or later (64-bit), or Macintosh OS X 10.11 (El Capitan) or newer versions


Screenshot of SutterPatch Software

 

TECHNICAL SPECIFICATIONS

Dimensions
dPatch: 19 in x 11 in x 3.5 in | 48.2 cm x 28 cm x 9 cm

dPatch Preamplifier: 7.6 in x 3.5 in x 1.2 in | 19.5 cm x 9 cm x 3 cm

dPatch Headstage: 3.9 in x 1.4 in x 0.75 in | 10 cm x 3.5 cm x 1.9 cm

Weight
dPatch: 15 lbs | 6.8 kg

Electrical
110/240 Volts
50/60 Hertz power line

*Patent No. US 10,393,727 B2

 

SYSTEM REQUIREMENTS

  • Minimum Configuration:
  • Windows 10 (64-bit), or MacOS: 10.11, El Capitan or later
  • Processor: Dual-core i5
  • Memory: 8 GB
  • Solid-state drive (SSD): 500 GB or greater
  • Display Resolution: 1024 x 768 (XGA)
  • 1 available USB 3.0 SuperSpeed port (on the main board, not a PCIx card or similar)

 

  • Recommended Configuration for Bandwidths of >50 kHz:
  • Windows 10 (64-bit), or MacOS: 10.11, El Capitan or later
  • Processor: Dual-core i5
  • Memory: 16 GB
  • Solid-state drive (SSD): 500 GB or greater
  • Display resolution 1920 x 1080 (Full HD)
  • 1 available USB 3.0 SuperSpeed port (on the main board, not a PCIx card or similar)

SUTTERPATCH® Data Acquisition Management System and Analysis Software: Included with all Sutter Instrument Amplifier Systems

Notes:
USB 3.0 ports are compatible with USB 2.0 High Speed specifications.
Slower USB 2.0 ‘full-speed’ ports, which are sometimes found on older Windows PCs or USB add-in cards, are not supported.

To check for High Speed USB 2.0 or USB 3.0 on a PC computer running Windows, look in the Control Panel > Device Manager > Universal Serial Bus controllers section for “Enhanced” host controllers. As this does not provide any mapping information to the computer’s physical ports, and there can be a mix of USB port versions, you should check individual USB ports for USB 2.0/3.0 High Speed operational performance. As a visual indicator, USB 3.0 ports are often color coded blue.

USB hubs are not supported. USB add-in cards, even if they formally meet High Speed or Super Speed specifications, are not recommended. They are often architecturally configured as USB hubs and may lead to intermittent transfer errors that are hard to troubleshoot.

Operating systems installed within virtualization software platforms such as VMware and Parallels are not supported.


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