膜片鉗電生理訊號放大器 Patch Clamp Amplifier

The Model 2400 is a low-noise, full-featured patch clamp amplifier designed for voltage clamping using patch electrodes on single channels or whole cells. Its design also allows fast intracellular current clamp measurements with sharp electrodes.

Amplifier current gain can be matched to your experimental needs by selecting one of the three available dual resistive feedback probes, as the gain is partially determined by the value of the resistor in the headstage. There are three heastages to choose from: 1 GΩ/10 MΩ, 10 GΩ/10 MΩ, and 10 GΩ/100 MΩ. The wide range of feedback resistors means currents can be recorded with outputs of 1 mV/nA to 10 mV/fA. The standard probe sent with each unit is the 100 M>Ω/10 G>Ω version, but other values can be selected.

Unlike other patch clamp amplifiers, the Model 2400 has a voltage follower in the probe. This allows this amplifier to be a true fast current clamp amplifier with no instability. An integrated four pole low pass Bessel filter provides flexible signal conditioning. Fine tuning capacity compensation is available to eliminate virtually all electrode-induced transients. Calibrated whole cell compensation provide easy display of membrane capacitance and access resistance.

A host of command potentials are integrated internally within the model 2400, including an automatic tracking command to zero the membrane current, manual controls for offset and holding potentials, and an easily readable digital display. For signals that are more complicated, an external command input with different scaling factors is available for use with any signal source. A digital meter provides accurate values of command signals and membrane currents or voltages, the true RMS noise of the amplifier and experimental setup, the cut off frequency of the low pass filter, and the overall gain of the amplifier plus probe.

Series resistance compensation provides the researcher with the option of introducing either or both predictive and corrective compensation from zero to 100%. Fine and coarse controls for lag provide sensitive control to minimize oscillation produced by compensation close to 100%. Separate compensation controls exist for eliminating transients seen during current clamp experiments when the bridge balance is used.

Telegraph outputs provide analog voltage equivalents of front panel settings including error conditions, amplifier mode and gain, Cmembrane, RMS noise, and low pass filter cut-off value. These telegraphs allow the Model 2400's front panel settings to be automatically recorded by your system software.

The Model 2400 Patch Clamp Amplifier from A-M Systems can serve as a high quality research amplifier in your laboratory, or an inexpensive teaching rig in your graduate courses . Please contact us for information about a free Model 2400 “test drive” in your own lab… No purchase required.

Probe Gain and Bandwidth

Probe Case Size

• 2.1" (54mm) long x 0.8"(20mm) high x 1.4"(36mm) wide
• BNC input connector
• 1 mm ground pin connector

Maximum Instrument Noise

Voltage clamp modes measured with the internal 4 pole Bessel filter and minimum external noise.

Current clamp modes have noise levels less than 20 µV with inputs shorted.

Internal Bandwidth

For voltage-clamping only the bandwidth is settable from 10 kHz to 100 kHz.

Membrane Voltage Output Range

• 10 mV/mV-1 V/mV

Filter

• 4 pole Bessel filter with 6 pre-set values, and bypass: 0.5 kHz, 1.0 kHz, 2.0 kHz, 5.0 kHz, 10.0 kHz, and 20.0 kHz.
• 0.1 kHz resolution

Capacity Compensation

• Voltage clamp:
  • FAST1: 0-10 pF, 0.2-2 µs
  • FAST2: 0-1 pF, 0.1-10 ms
• Current clamp
  • FAST1: 0-10 pF
  • No FAST2

Whole Cell Compensation

• Raccess: 0-100 MΩ
• Cmembrane: 0-100 pF

Series Resistance

• RsPre: 0-100%
• RsComp: 0-100%
• LagCoarse: 1-100 µs
• LagFine: 1-10 µs

DC Balance

• Up to the value of the low feedback resistor in the probe

Hold Command

• Voltage clamp: ±200 mV
• Current clamp:
  • Probe Resistor: 10 M / 100 M
  • Command Range: ±200 nA / ±20 nA

Offset Command

• Voltage clamp: ±100 mV
• Current clamp: ±1 V

Speed Test Command

• Used in voltage clamp only.
• Probe High Gain: 0.2 nA p-p at 60 Hz
• Probe Low Gain: 1 nA p-p at 60 Hz

Vcomp Command

• ±5 mV at 60 Hz

Iresist Command

• ±1 nA at 60 Hz

External Command

• Voltage clamp: 100 mV/V with the external switch set to divide by 10, 20 mV/V with the external switch set to divide by 50
• Current clamp:
  • Probe Resistor: 10 MΩ / 100 MΩ
  • /10 Range: ±100 nA/V / ±10 nA/V
  • /50 Range: ±20 nA/V / ±2 nA/V

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