單一肌細胞收縮力測量系統 MyoStretcher System
- 準確的舒張鈣離子 Accurate diastolic calcium
- 等張和等長收縮力 Auxotonic and isometric contractions
- 長度相關刺激 Length-dependent activation
- 力量速度關係 Force‐velocity relationship
- 單一細胞收縮循環 Workloops in single cells
MyoStretcher 可以作為獨立系統，也與現有 IonOptix 肌細胞收縮與鈣離子測量系統結合。
IonOptix-configured inverted microscopes provide an ideal platform for combined photometry and dimensioning measurements. These microscopes have been engineered to incorporate various unique IonOptix optical components and connectors in order to operate as the imaging component within our Calcium and Contractility Systems. Choose between the Motic AE31 or the Olympus IX73 depending on your application.
The fluorescence excitation light source is chosen with your specific research needs in mind and can be configured for almost any dye or combination of dyes. Options include the ultra-fast HyperSwitch, monochromatic LEDs, or the more affordable MuStep.
The sensors within the Calcium and Contractility system are responsible for translating cellular signal into data. Cell contractility is measured using the high-speed MyoCam-S3 digital camera, and whole-cell fluorescence photometry is measured with a photomultiplier tube (PMT). For cleaner fluorescence signals, the Cell Framing Adapter allows you to frame a cell of interest and reduce background fluorescence.
The field stimulator is an important component of excitation-contraction studies to provide acute stimulation of cells. Use either the MyoPacer for simple stimulation protocols or the MyoPacer EP for advanced sequencing and arrhythmic stimulation.
The FSI ties the Calcium and Contractility system components together by communicating between hardware and the computer, allowing for synchronous control of contractility and/or calcium measurements as well as inputs and outputs for external devices.
Cells must be maintained in an appropriate chamber environment during experiments. For MyoStretcher systems, a rotational version of the FHD chamber is available that makes it easier to orient cells for mechanical loading.
For simple MyoStretcher configurations, an economical rail option is available that is compatible with most microscope types.
Stable Post Configeration
For experiments where stability is essential, such as work loops, the stable post configuration provides an extremely secure platform for your MyoStretcher measurements.
Designed as a core part of our MyoStretecher system, the IonOptix OptiForce is a revolutionary optical fiber interferometry-based force transducer specially designed to detect the nanoscopic forces from single isolated cardiac cells. It offers two major advantages over existing technologies: sensitivity and speed, with detection limits down in the low nanoNewtons and a frequency response above 3kHz.
With the piezoelectric motor for length control, you can specify cell length changes as percentage or absolute changes and use programmable wave to wave amplitude and duration changes to customize the stretch waveform.