MEA2100 Mini 簡易型微電極陣列電生理訊號記錄系統

• 架構簡單、輕便的電生理系統，甚至比標準的MEA2100更加輕便
• 可兼容於大部份的顯微鏡與微操作手臂設備
• 使用可重覆使用*的微電極陣列晶片(MEA)進行實驗，可視實驗需求更換不同種類的晶片
• 系統擁有60頻道與120頻道的版本**
• 可擴充至最多8頻道，一次收集來自8片MEA的電生理訊號
• 包含Multichannel Suite 控制/數據收集軟體
• 可以使用大部分MEA2100系統之配件，例如溫控系統或是灌流系統

* 可重覆使用次數視使用情形而定，每次使用完畢亦需要參照標準清潔程序進行清潔

** 微電極陣列晶片無法與不同頻道數之系統兼容

The MEA2100-Mini-System is a miniaturized in vitro recording system. The state of the art power saving electronics enable the option to operate the system continuously in an incubator while maintaining most of the MEA2100 platform advantages. The system consists of a 60- or 120-channel headstage, featuring a sampling rate of 50kHz per channel at a 24bit resolution, as well as a 2-channel stimulator with user customizable patterns. The digitized data is transfered from the headstage to a signal collector unit for up to four headstages. The signal collector unit is connected via an eSATA cable to the interface board.  With a multifold MEA2100-Mini-System up to two signal collectors can be connected to one interface board, thereby creating a system with 8 headstages for parallel recordings.

IFB-C

As part of the system, the IFB-C multiboot is a new interface board generation which is able to receive data from a MEA2100(-Mini)-Headstage, a Multiwell-MEA-Headstage, a CMOS-MEA5000-Headstage, a MEA2100-Beta-Screen-Headstage, a ME2100-Headstage, or a W2100-Receiver. This makes cost-effective combinations with only one interface board and multiple recording systems possible. You can establish for example your experiments on 60, 120, or 256 electrodes with the MEA2100-System, then add the Multiwell-MEA-Headstage and start high-throughput screening. It's also possible to use the same interface board for in vitro MEA experiments and tethered / wireless in vivo recordings. This leaves you with even more flexibility in switching between possible configurations for your specific research needs.

Key features:

• Real-time signal detection and feedback*
• Freely programmable DSP*
• Multiple inputs/outputs, including digital, analog, and audio
• SuperSpeed USB 3.0 ports

* applicable for MEA2100(-Mini), ME2100, and W2100

MEA2100-mini Setup

The MEA2100-Mini-System consists of several components: MEAs, headstage, signal collector unit, interface board, and PC with software. Optionally, you can add temperamture controller, as well as an perfusion cannula and peristaltic perfusion pump. Considering the small size of the headstage, the system facilitates recordings in areas of restricted size (e.g. on microscopes) or inside a humid incubator. Please click on the arrows on the right to learn more about the single components.

MEAs

The recording process starts at the MEA, the microelectrode array with embedded electrodes. You can culture your cells directly on the MEA or place your slices on it. Normally, it is equipped with a ring, which serves as a culture chamber.

The planar electrodes with a dimension of typically 10-30 µm are arranged in a square recording field where the spacing between these electrodes is in a range of 30-700 µm. The electrodes are embedded in glass substrate with a size of 5x5 cm.

Multi Channel Systems offers the widest variety of MEA layouts. The MEA2100-Mini-System can be used with all MEAs with 60 electrodes or 120 electrodes respectively. You find the complete range of MEAs here.

Headstages

The headstage is the core element of the system. It is designed with a very small footprint, so you can record from slices or cell cultures even if space is a limited factor in your setup. Due to state-of-the-art power saving electronics, the headstage can be operated in an incubator for continuous recordings in a controlled environment.

The headstage of the MEA2100-Mini-System features either 60 or 120 recording channels. The recorded signals are amplified and digitized at a 24bit resolution directly on the headstage, thereby optimizing the signal-to-noise ratio.

Each headstage offers two independent stimulation channels. The stimulation patterns can be customized via the included Multi Channel Experimenter software.

Signal collecting unit (SCU)

The signal collector unit gathers all data from the headstages and transfers them to the interface board. You can connect up to 4 headstages to each SCU, while 2 SCUs can be connected to one IFB. The signal collector unit can also control up to four optical stimulation units including high power LED.

Inteaface board

The interface board receives data from the headstage via a iX industrial type B cable. In the interface board, you find a freely programmable digital signal processor, which can be used for real-time signal detection and feedback. Moreover, you find various analog and digital in- and outputs for synchronization with other instruments. The interface board connects to the computer via USB 3.0 SuperSpeed. The IFB-C is a new interface board generation which is able to receive data from MEA2100-Headstages, Multiwell-MEA-Headstage, CMOS-MEA5000-Headstage, ME2100-Systems or W2100-Systems. This makes cost-effective combinations with only one interface board and multiple recording systems possible.

Higher channel count with 8 headstages

The MEA2100-Mini-System offers the possibility to record from up to 8 headstages.

You can connect two signal collector units to one interface board. Each signal collector unit can receive data from up to four headstages. Thereby, you can build a system with 8 headstages, recording from 480/960 channels.

The interface board and signal collectors are equipped for this high channel count from the beginning. This way, you can start with one headstage (60 or 120 channels) and add-on more as you go. This gives you the flexibility to adjust the system to your changing experimental needs.

Computer and software

It consists of three programs and updates are available for free here.

• Multi Channel Experimenter is the data acquisition and online analysis tool. With an easy-to-use drag’n’drop interface, you simply create your virtual experiment with e.g. data source, filters, spike detection, and recorder. The software indicates the battery level and signal quality and displays the data in real-time.
• Multi Channel Analyzer is the offline analysis tool. You just import the data recorded with the Multi Channel Experimenter and then define your in-depth analysis with more instruments. The Multi Channel Analyzer also supports synchronized video input. This is an advantageous feature when doing behavioral research with freely moving animals. The video data and electrophysiological recordings are displayed in parallel and you can easily skip through the data from one event to the next.
• Multi Channel DataManager facilitates the data export for analysis with third-party programs. It supports all files generated with the Multi Channel Experimenter and then exports the data into HDF5 (e.g. Matlab, Python), .nex (Neuroexplorer) or .ced (Spike 2). Independent of the file format, the export is done quickly and a data compresssion is included.

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