Vutara VXL 超高解析度顯微鏡工作站

• SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
• Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications

• Epi-fluorescence microscopy with large FOV
• Transmitted light microscopy with large FOV

• 405 nm, 120 mW

• 488 nm, 2000 mW (optional)

• 555 nm, 2000 mW

• 638 nm, 1200 mW

• 750 nm, 1500 mW (optional)

• Flat excitation profile guaranteed by top hat illumination from a square fiber

• Up to 5 colors sequential

• 2 colors simultaneously with 2 cameras (optional)

• Simultaneous imaging of two focal planes allows 1 µm depth discrimination (larger z range possible in z-stack mode)

• Orca Flash 4.0 V3 sCMOS camera

• Orca Fusion BT sCMOS camera (optional)

• 60x magnification

• 1.3 numerical aperture (NA)

• Silicon oil immersion

• 0.3 mm working distance

• #1.5H cover glass (0.170 ±0.005 mm)

• 200 µm x 200 µm for multiplexed localization microscopy and widefield imaging

• 50 µm x 50 µm for SMLM with switching (STORM, PALM, PAINT) and 3D localization (biplane detection)

• Larger FOV with tile scanning

• Fitting of measured PSF to maximize accuracy in xyz

• Proprietary biplane for high accuracy z-resolution and deep sample imaging

• Better than 20 nm laterally (xy)
• 50 nm axially (z) with biplane

• > 50 µm (typical, dependent on sample)

• SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
• Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications

• Course focus to localize sample
• Fine focus for fast z-stack acquisition

• Active focus drift correction during data acquisition
• Focus drift <30 nm over 10 minutes
• xy drift correction post-acquisition

• Live cell incubation with humidity, CO2, and temperature control (optional)

• Multiplexed exchange of sample buffer (optional fluidics unit)

• 15 samples + buffer x 2 ml reservoirs for imaging probes

• 96 samples + buffer

• Seamless integrations in SRX microscope control software

• Space required if microscope and laser launch are installed on same table: 120 cm x 75 cm (4 ft x 2.5 ft)

• Microscope without camera:

                  34 cm x 40 cm x 34 cm, (1.2 ft x 1.3 ft x 1.2 ft)

                  35 kg (78 lbs)

• Laser launch:

                  33 cm x 53 cm x 32 cm (1.0 ft x 1.7 ft x 1.0 ft)

                  30 kg (65 lbs)

• Electronics box stacked on top of laser launch:

                  53 cm x 35 cm x 14 cm (1.7 ft x 1.1 ft x 0.4 ft)

                  8 kg (17 lbs)

• The light-tight design does not require dimmed room light

• The system includes vibration insulation (sturdy table or bench required for installation)

• Optional: Customers can supply optical table for challenging environments

• SMLM modalities

                  STORM / dSTORM

                  PALM

                  PAINT

                  Other blinking-based modalities

• Genomics modalities

                  Chromatin tracing

                  smFISH

• Multiplexing workflow

                  Tracking of probe cycles and positions

                  Support for an unlimited number of probes

• Calibration workflow to ensure superior localization precision

• xy fitting based on measured point-spread function (PSF)
• z position determined from calibrated biplane data
• Initial localization during data acquisition
• Statistical tools to validate localizations

• Diffusion analysis based on nearest neighbors

• Particle assignment based on sparse emitter tracking

• Mean-square displacement and distribution analysis and plots

• Mean velocity and angle calculations and plots

• Setup and control multiplexed imaging workflow
• Tracking of probe labels and metadata

Drift correction

• Auto-correlation
• Fiducials embedded in the sample

• Spatial distribution

                  Ripley’s K functions

                  Pair correlation

                  Nearest neighbors

• Cluster analysis

                  DBSCAN

                  OPTICS

                  Delaunay

                  Particle counts

                  Volume and density calculations

                  Determination of radius of gyration and sphericity

                  Principal component analysis (PCA)

                  Calculation of centroid

• Colocalization

                  Pearson’s correlation coefficient

                  Mander’s overlap coefficient

                  Intensity correlation quotient

                  Cross pair correlation

                  Joint histogram

                  STORM-RLA

• Resolution analysis

                  Fourier ring correlation

                  Labeling density resolution analysis

                  Local resolution correlation mapping

                  Nearest neighbors’ analysis

• Optical reconstruction of chromatin architecture

• 2D and 3D visualizations
• Point cloud and point splatting
• Volumetric slices
• Isosurface
• Maximum intensity rendering
• Depth color coding
• Time frame color coding
• Color coding by probe
• Cluster hull visualization
• Wireframe visualization of clusters
• Trajectories for tracked particles

• Raw images are stored in highly efficient binary format and can be exported in ome-tiff format

• Export of drift corrected data

• Localizations are stored in highly efficient binary format and can be exported as .csv or MATLAB binary files

• Results from cluster analysis are stored and can be exported

• Export of results from statistical analysis as .csv or MATLAB binary files

• All imaging and processing settings are stored in XML or JSON format

• Images can be exported as vector graphics or user-defined size/DPI

• Specifications for all data formats are published

• 90 TB effective storage

• 2 Gb/sec transfer rate