aCSF人工腦脊髓液即溶包

 [最新] 含有蔗糖以及不含任何鈣/鎂離子的的aCSF即溶配方現已上市

• 標準版 
• 含蔗糖版  (適合用於急性組織切片復原, 例如使用震盪切片機進行腦切片)
• 不含鈣鎂離子版  (讓您自行控制鈣鎂離子的濃度, 進而完全控制離子通道活性)

• 使用預先配好的單獨 aCSF 粉末包可節省大量的時間-每包粉末幾秒鐘即可溶解，且無需額外添加 Mg2+ 或 Ca2+調整酸鹼值。
• 每包粉末的配方都相當準確，提供實驗更高的再現性-減少誤差，獲得更好的數據。
• 可在常溫環境下保存的密封獨立包裝，沒有受潮問題
• 經過多種電生理實驗的廣泛驗證。

* 配製好的溶液仍需使用氧氣/二氧化碳混合氣體通氣給氧

Figure 1. Representative traces of different experiment types carried out in aCSF Instant Powder (packets) (HB9200)

Representative traces from four different experiments to measure NMDA dependent plateau potentials, excitatory post synaptic potentials (EPSPs), inhibitory postsynaptic currents (IPSCs) and excitatory postsynaptic currents (EPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314 and stimulated via the stratum radiatum to excite Schaffer collateral synapses.

Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.

Figure 2. Spontaneous EPSC and IPSC currents recorded using aCSF Instant Powder (packets) (HB9200)

Traces showing both spontaneous excitatory postsynaptic currents (EPSCs) and spontaneous inhibitory postsynaptic currents (IPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314. Neurons were held in voltage clamp with sEPSCs being recorded at -70mV and sIPSCs being recorded at 0mV.

Figure 3. Long term recording of EPSCs in aCSF Instant Powder (packets) (HB9200) for over 70 minutes

Summary of experiment recording excitatory post synaptic currents (EPSCs) for over 75 minutes in mouse pyramidal neurons. Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314, held at -70mV in voltage clamp and stimulated in a paired pulse stimulation protocol (50ms interval) via the stratum radiatum to excite Schaffer collateral synapses. Figures shown are representative traces from different timepoints during the experiment, the input current required to hold the cell at -70mV, the series resistance, the amplitude of the initial EPSC and the ratio of amplitudes of the two EPSCs (paired pulse ratio).

Figure 4. aCSF Instant Powder (packets) (HB9200) manufactured by Hello Bio

Simply add to 1L of dH2O and mix to create a high quality electrophysiologically validated aCSF

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參考資料：

Reduced expression of the psychiatric risk gene DLG2 (PSD93) impairs hippocampal synaptic integration and plasticity.

The development of synaptic plasticity induction rules and the requirement for postsynaptic spikes in rat hippocampal CA1 pyramidal neurones.