Passive / Active Avoidance Box: PACS-30 主/被動迴避行為測試箱
PACS-30迴避行為測試箱可用來進行主動迴避與被動迴避實驗，箱內包含了燈光與聲音刺激，可用來給予條件刺激(Conditioned stimulation, CS)以及電擊刺激，可用來給予無條件刺激(Unconditioned stimulation, US)。實驗步驟及刺激強度皆可控制且大小鼠通用。
PACS-30 is an automated system employed in the testing of avoidance behavior. Each subject is housed within a cage that has been divided by a centrally located wall. The wall has an opening that allows the subject to pass between the compartments. The lid of each compartment contains a light source. The acoustic source is on the center divider. The subject walks on a shocker grid assembly that spans the entire floor area of both compartments. A series of photocells detects the subject position within the cage.
When configured for active avoidance, PACS-30 presents a user-programmed sequence of stimuli to the subject. In response, the subject must relocate to the adjoining compartment within a preset time in order to avert the presentation of a mild shock. Controlling software coordinates stimulus presentation and behavioral responses in up to 30 avoidance cages. Latency from stimuli onset to transfer is recorded to a data file.
When configured for passive avoidance, each PACS-30 avoidance cage is equipped with an automated door that prevents inter-compartment transfers. A similar regime of stimulus presentation is sequenced during which the animal is offered the freedom to transfer to the adjoining compartment. Failure to transfer indicates success in the establishment of passive behavior. An animal that fails may be presented with a mild punishment.
The controlling software also incorporates features that allow the establishment of parameter files. A parameter file contains all controlling aspects of stimuli presentation (start times, durations and intensity, etc.) easing the set-up of subsequent tests that will follow a common testing protocol. PACS-30 allows independent control of up to 30 avoidance cages. Advanced features include the ability to delay the trial until after a preset amount of exploration time has lapsed and punishment for inter-trial transfers.
Performs both Active and Passive Avoidance Testing Supports operation of up to 30 boxes Standard visual and acoustical stimuli Cage made of Makrolon
Avoidance Cage Dimensions: 19"L x 9"W x 10.75"H (48cm x 23cm x 27cm) Motion Detection Method: Multiple Infrared Beams
Cage Material: Makrolon & Stainless Steel
Stimulus Voltage: 162 to 200 volts
Shock Method: True Constant-Current, 100ms pulse every 250ms (4Hz)
Shock Current: Range of 0-1000uA. 11 fixed set-points (0-10) factory selected at: (0, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000uA) automatic calibration.
Stimulus Current Regulation: 1% for 0-163 KOhms.
Sound Frequency: Range of 200-13,000Hz in 100Hz steps with automatic calibration
Sound Volume: Range of Mute-115dB. 11 adjustable set-points (0-10) factory selected at: (Mute, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115dB) adjustable ±3dB
Lamp: Phillips Luxeon K2 LED, warm white output from 0-150Lux
Lamp Intensity: Range of 0-150Lux. 11 adjustable set-points (0-10) factory selected at: (0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150Lux) adjustable ±10Lux