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全自動膜片箝定系統 Automated Patch Clamp

產品型號:
廠牌名稱: Multichannel Systems
PatchServer是全球唯一可操作單一離子通道及全細胞電位紀錄(Whole cell recording)的自動化膜片箝定系統。其技術設計及工具配件使用皆源自於傳統手動操作方法。
PatchServer以傳統手動操作為基礎而增設其配件。在記錄電極部分採用標準玻璃電極,操作步驟設計上皆與傳統操作法相同。然而PatchServer可進行全自動操作,從細胞挑選到數據收集皆無需人力,大大的降低了細胞電生理實驗的技術門檻!

產品特點

Logo PatchServer

Pipette Based Automatic Patch-Clamp System

PatchServer full setup

The PatchServer is world-wide the only automatic patch-clamp system that is able to establish single-channel and whole-cell recording configurations using tools and techniques from the manual patch approach. The PatchServer is an add-on tool for manual patch-clamp setups. It utilizes standard glass electrodes and employs step-by-step the manual patch-clamp procedure of a human experimenter.

  • PatchServer specific components
    Specific PatchServer parts are added to a manual patch-clamp setup to form a fully functional PatchServer
  • Fully automatic patch-clamp procedure with standard glass electrodes
    The PatchServer utilizes tools and techniques of the manual patch approach
  • All suspend-able cell types can be patched
    Every cell type with a minimum diameter of ~10 µm and that can be brought into solution, can be patched by the PatchServer
  • Modular construction
    Depending on customer need, MCS provides equipment from the minimal PatchServer specific parts to a complete, fully functional patch-clamp setup

The PatchServer can patch a cell and establish the requested recording configuration fully automatic without any user interference. If desired, the user has the option to online interact with the patch process.  The user can fine-tune and supervise the patch process using controls within the PatchServer software with a seamless range of control from “not interfering - automatic” to “full manual control”.

Due to the 4 pipette holder array, the PatchServer increases the data production rate compared to a manual patch-clamp setups and its pipette based patch approach guarantees low operating costs. Compared to other automatic patch-clamp systems, the PatchServer is the only system that allows the user to identify the cells before they are used for an experiment.

  • Whole-Cell and single-channel recording configurations are possible
    The PatchServer can automatically established all four recording configurations
  • Simultaneous, individual 4-channel recordings
    The four pipette holder array supports the simultaneous data acquisition of 4 individual experiments
  • Low operating costs
    Using standard glass capillaries eliminates the need for expensive consumables
  • After random catch, the cell can be visually identified before it is patched
    The user can decide if a cell should be used or discarded by the PatchServer
  • Consistent high data quality combined with permanent quality control
    Automatic patch approach and software algorithms guarantee constant high data quality level
  • Ultra-fast application tool
    Add-on tool for the PatchServer that automates the accurate measurement of fast-desensitizing ion currents

 

產品規格

One-channel PatchServer setup for automatic establishment of any patch clamp recording configuration on suspended cells. Includes main unit, platform, catch pipette with amplifier, software, data acquisition computer with preinstalled software, and accessories.

PatchServer specific parts
  • Main Unit
    - 3 pressure controllers (1 for recording pipette, 1 for catch pipette, and 1 for cell reservoir)  
    - Power supply (100 - 240 V)
    - Possibility to upgrade to ultra-fast-application tool or 4-channel version
  • Platform
    - Fixed platform for inverted microscopes  
    - Mounting plate with fixed bolt-down positions for cell reservoir, catch pipette and ultra-fast-application tool
    - Height adjustable legs
  • Bath Chamber
    - Elongated main bath compartment
    - 4 individual application bays, each with separate inline
  • Cell Reservoir
    - Cell storage container with integrated special trituration tube
    - Capillary for cell delivery to catch pipette
    - Coarse manipulator (LBM-7, Scientifica) with step-motor for trituration
  • Catch Pipette
    - Catch pipette amplifier with housing and tiltable stand
    - Holder for catch pipette
  • PatchServer-1 Software
    - Automatic control & supervision of entire patch procedure (1 channel)  
    - Programmable protocol assays & fully automatic execution
    - Unrestricted user interrupt possibility & switch to manual patch procedure
    - Interface to PATCHMASTER (HEKA)
    - For Windows 8 or 10

Data Acquisition Computer:
  - PC with 1 monitor, keyboard and mouse
  - With preinstalled hardware operating system and software

Micromanipulator from Scientifica Ltd (PatchStar)
    - Motorised 3 axis super smooth micromanipulator
    - 20 mm of high resolution (20 nm) travel in XYZ and fourth virtual approach axis
    - Drift of less than 1 micron over two hours
    - Extremely low electrical noise

Motorized Stage (UMS-2500-00) for microscope: Scientifica, high capacity
      XY stage, ultra-low noise 3-axis controller

Perfusion Pump
     2-channel peristaltic perfusion pump (PPS2 from MCS)

Technical specifications as specified in the data sheet unless noted otherwise in the order.

Please note: You need additional devices (e.g. microscope and anti-vibration table) to operate the PatchServer-Basic. Please contact your sales representative for a detailed list.

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